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Objective To explore the relationships between inappropriate parenting styles,emotional regulation strategies and self-injury.Methods A total of 1 096 medical students anonymously completed adolescents self-harm scale,emotion regulation scale (ERS) and the rejection and over-protection subscales of parental bonding instrument (PBI).Results The scores of paternal rejection,maternal rejection,paternal over-protection,maternal over-protection,cognitive reappraisal,expression suppression and self-injury were (4.3±3.3),(3.9±3.0),(5.7±3.3),(6.1±3.5),(36.1±6.7),(25.7±7.4) and (1.0±2.9),respectively.The scores of self-injury were positively correlated with the scores of paternal rejection,maternal rejection,paternal over-protection,maternal over-protection and expression suppression (r =0.08-0.20,P＜ 0.01),meanwhile negatively correlated with the scores of cognitive reappraisal(r=-0.10,P＜0.01).Cognitive reappraisal mediated the relationship between parental rejection and self-injury,which accounted for 9.3％.Expression suppression mediated the relationship between parental rejection,parental over-protection and self-injury,which accounted for 14.5％ and 17.9％,respectively.Conclusion The emotion regulation strategy act as a mediator between the inappropriate parental styles and self-injury in medical students.In addition,different emotion regulation strategies (cognitive reappraisal and expression suppression) play different roles in such relationship.
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Objective To investigate the expression and diagnostic significance of DOG1,CD117and CD34 in gastrointestinal stromal tumors.Methods Expressions of DOG1,CD117 and CD34 were determined by immunohistochemical techniques (EnVision) in 60 cases of gastrointestinal stromal tumors (GIST) and 14 cases of other mesenchymal tumors.Results Expression of DOG1 in GIST was significantly higher than in other mesenchymal tumors (P =0.000).Expression of DOG1 in GIST from stomach was significantly higher than that from intestines.The expression rates of DOG1,CD117 and CD34 in GIST were 95％,95％,and 83.3％,respectively.Expression of DOG1 was significantly higher than CD34 (P =0.040),but no significant difference between DOG1 and CD117 (P =1.000).The expression rates of DOG1 in CD117-positive,CD34-positive tumors were 94.7％ and 94.1％,respectively.All the CD117-negative,CD34-negative tumors expressed DOG1,3 cases which were negative for CD117 and CD34 expressed DOG1.Conclusions DOG1 is a novel marker for GIST,It is necessary to the combined detection of the expression of DOG1,CD117 and CD34,which will further improve the diagnostic accuracy of GIST.
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<p><b>OBJECTIVE</b>To investigate the reliability of magnetic-activated cell sorting (MACS) combined with multiplex ligation-dependent probe amplification (MLPA) in the detection of the molecular cytogenetic abnormalities in multiple myeloma.</p><p><b>METHODS</b>The bone marrow cells were collected from 29 patients with multiple myeloma. The immuno magnetically sorted and unsorted cells were detected for TP53 and RB1 expressions using MLPA probes and the results were compared with fluorescent in situ hybridization (FISH).</p><p><b>RESULTS</b>The detection success rate was 100% for MLPA, which yielded results with an concordance rate of 99.1% with the FISH results. The positivity rates of MLPA and FISH were both increased after immunomagnetic sorting of the bone marrow cells.</p><p><b>CONCLUSION</b>MLPA can well suit the clinical needs for detecting molecular cytogenetic abnormalities in multiple myeloma, and the samples should be immuno magnetically sorted before the assay.</p>
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Chromosome Aberrations , Immunomagnetic Separation , In Situ Hybridization, Fluorescence , Karyotyping , Multiple Myeloma , Diagnosis , Genetics , Multiplex Polymerase Chain ReactionABSTRACT
Objective To investigate and understand the characteristics and drug intervention of post-traumatic stress symptoms(PTSD) among middle age acute leukemia patients.Methods The Essen Trauma-Inventory (ETI) was administrated to 100 middle age acute leukemia patients who were admitted in our department and study the PSTD symptoms.Results The positive rate of PTSD is 21.21％ in sample of the patients.Total PTSD symptoms scores were (18.10 ± 9.42),there is no significant different between the 3 main PTSD symptoms in these patients(P ＞ 0.05).Type of leukemia,sexuality,levels of education and marital status are influencing factors of PSTD symptoms,there is significant different between the subgroups (P ＜0.01).Drug intervention can significantly relieve PTSD symptoms in these patients (Z =13.56,P ＜ 0.01).Conclusions Middle age acute leukemia patients are generally found PTSD symptoms and with a high incidence rate of PTSD,so it needs timely diagnosis and treatment.
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Objective To investigate the expression of surfactant protein A(SP-A)in adenocarcinoma and squamous cell carcinoma of lung and its clinical significance.Methods The expression of SP-A was determined by immunohistochemical assay (EnVision) in 27 cases of adenocarcinoma,15 cases of squamous cell carcinoma of lung.Then the association of SP-A expression with clinicopathological parameters was analyzed.Results The expression rate of SP-A was significantly higher in adenocarcinoma of lung than that in squamous cell carcinoma of lung(51.85% vs 6.67%,P<0.01).The expression rate of SP-A in papillary adenocarcinoma,bronchioloalveolar carcinoma,acinar adenocarcinoma and musinous adenocarcinoma of lung was respectively 85.71%,50%,40%and 25%.There was no correlation between the expression of SP-A and gender,age,tumor size and lymph node metastasia.Conclusion The expression of SP-A may be associated with histological types of the lung carcinoma,which may be used as important marker in differential diagnosis of adenocarcinoma and squamous cell carcinoma of lung.